| 1. | Bacterial artificial chromosome ba
细菌人工染色体 |
| 2. | Yeast artificial chromosome ya
酵母人工染色体 |
| 3. | Construction and characterization of a dunaliella viridis bacterial artificial chromosome library
细菌人工染色体文库的构建和鉴定 |
| 4. | In order to construct plant artificial chromosome , plasmid vectors have been constructed to integrate necessary elements into both right and left yac arms
为了构建植物人工染色体，我们构建了可以和含有着丝粒序列的yac载体的左臂和右臂进行同源重组的质粒载体。 |
| 5. | The successfully constructed artificial chromosome would also can be used as a new vector to introduce chromosome segments of hundreds kb to mb length into plant cells
成功构建的植物人工染色体也可以作为一种新型的载体用来克隆和转化长达几百kb到一个mb的外源dna进入植物细胞。 |
| 6. | To research melanin synthesis and its accumulation mechanism , we constructed a silky bacterial artificial chromosome ( bac ) library . the library was used to study the two genes on chicken chr
为研究丝羽乌骨鸡体内黑色素合成和沉积的分子机制，本研究构建了丝羽乌骨鸡的基因组细菌人工染色体( bac )文库。 |
| 7. | The construction and introduction of plant artificial chromosomes into plant cells would be an effective way to understand the essential elements necessary for maintaining chromosome function and structure in plants
1997年，美国的casewesternreserve宣布构建成功第一代hac 。在植物细胞中构建人工染色体将为植物染色体的结构和功能以及染色体的各必须组分的研究提供有效的工具和手段。 |
| 8. | It have been reported that the 210 kb yeast artificial chromosome ( yac ) including the human a - lactalbumin gene could direct the transgene to express in a position - independent manner , therefore is an excellent expression vector to produce transgenic animal bioreactors
根据报道含完整人-乳白蛋白基因的yac载体能指导外源基因的乳腺特异性高效表达，是生产动物乳腺生物反应器的理想表达载体。 |
| 9. | Isolation and purification of modified yacs from yeast strain have been carried out by pulsed - field gel electrophoresis . the modified yacs are expected to generate plant artificial chromosome after being transferred into arabidopsis protoplast by liposome - mediated method
用脉冲电泳将经过同源重组的修饰的yac克隆分离出来通过脂质体转化植物的原生质体，希望得到在植物细胞中稳定存在的植物人工染色体。 |
| 10. | The optimum parameters , such as the exact time for the activated eggs finishing the first chromosome sets copying , the optimum period for artificial chromosome set duplication , the optimum temperature and intensity of heat shocking etc , were determined
确定了在一定孵化温度下，被激活的草鱼卵子完成第一次染色体复制的准确时间，实施人工染色体加倍处理的最佳时机，人工加倍处理的最佳方法和处理强度等。 |
